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Title: Endodontic Microbiological Flora in Genetically Disordered Patients with Non-vital Unexposed Pulps (In vivo Study)
Authors: Hassib N.F; El-Badry T.H; Ali M.A; Kataya M; Ramzy M.I; El-Hilali G
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Keywords: Bacteria; Ectodermal dysplasia; Enamel hypoplasia; Root-canal
Abstract:This study was conducted to delineate different microorganisms in non-vital unexposed pulps
from 41 samples divided into two groups. Group A: normally developed teeth and group B: abnormally
developed teeth. The culture method by Microscan Walkaway and molecular method by polymerase chain
reaction were revealed for bacterial detection. 64 different bacterial species were detected from this study,
represented 28 different species, recovered from 17 different genera. The gram –ve rod shaped bacteria
predominated. The most prevalent bacteria found were anaerobic followed by aerobic and finally,
facultative anaerobes. The most found bacteria of group A were Staphylococcus, Fusobacterium,
Porphyromonas, Klebsiella, Eubacterium, and Prevotella, while in group B, they were Staphylococcus,
Bacteroides, Acidaminococcus, Prevotella, and Dialister. There was a difference between the microbiota
present in the two groups, two noval endodontic microorganisms Acidaminococcus and Morganella were
detected in group B of this study. Further studies should be done to determine the role of these two noval
endodontic bacteria in causing endodontic infections.