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Title: Isolation and Identification of Ice-Nucleating-Active Bacteria and their Antagonistic Bacteria from Pistachio Trees in Rafsanjan Region
Authors: Mahdieh Rostami
Aff: Department of Agriculture , Rafsanjan Branch, Islamic Azad University, Rafsanjan, Iran
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Keywords: Ice Nucleation Activity Bacteria; Pistachio; Frost Injury
Abstract:Sudden temperature decrease in the months of February, March and April when the trees are in the stage of being in buds, blossoms and green fruits will cause serious damage to the pistachio trees. One of the factors which increases frost injury, is ice nucleation activity (INA) bacteria that produces biological ice-nuclei and it leads to causes frosting in higher temperatures. In this research, 6 cultivars of pistachio trees are selected. Samples of flower buds, leaves and green fruits were collected from Pistachio trees. On the basis of biological and physiological tests the isolated bacteria were identified as; Pseudomonas syringae Pseudomonas viridiflava, Pseudomonas fluorescens ,Pantoae agglomerans and Xanthomonas spp. Then in order to separate epiphytic antagonists of INA bacteria, all of the bacteria that were isolated from the surface of the plants, were individually examined with Pseudomonas syringae which has the most ice nucleation activity. All bacteria were tested for the production of compounds inhibitory around P. syringae on King’s B and nutrient glucose agar media. Antagonistic activity of Bacillus pumilus was identified and its activity on inhibition corn frost injury was investigated. Inoculum was applied from strain of B. pumilus at the rate about 108 cell/ml suspension on corn plants, but the frost injury on corn treated with antagonist bacteria did not differ significantly compared to untreated control.