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Title: Seasonal Variation in DPPH Scavenging Activity of Mentha Piperita
Authors: Daniela Grulová; Pavol Labun; František Šeršen; Ivan Šalamon
Aff: Department of Ecology, Faculty of Humanities and Natural Sciences, Presov University in Presov, 01, 17th November St., SK-08116 Presov, Slovak Republic
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Keywords: Peppermint; Mentha .piperita; DPPH; Antioxidant Activity
Abstract:Peppermint, the sterile tetraploid hybrid of Mentha aquatica and Mentha spicata, is commonly cultivated for commercial production of the essential oil, the drug Menthae piperitae folium, and the fresh herb. In this study, methanol extracts of Mentha . piperita leaves were screened for its antioxidant properties. Samples of leaves were collected in 2010 and 2011 from field cultivated plants in different development stages. Radical scavenging activity was determined spectrophotometrically by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and expressed as SC50 values. The highest antioxidant activity (SC50 = 10.11 - 57.35 .g/ml) exhibited leaves collected at the stage of flowering i.e. June – August