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Title: Degradation of Crude Oil in the Presence of Lead (Pb) and Cadmium (Cd) by a Metal-Adapted Consortium Culture
Authors: Kok-Kee Wong; Brid Quilty; Salmijah
Aff: School of Biotechnology, Dublin City University, Dublin 9, Ireland
Author Email:
Keywords: Consortium Culture; Biodegradation; Biosurfactant; Crude Oil; Heavy Metals.
Abstract:A consortium culture (CC) which was adapted to heavy metals was able to degrade 1% (v/v) of crude oil and after 8 weeks of incubation, no detectable peaks were found when analysed using GC-FID. There were significant decrease (p<0.05) in the surface tension (dynes/cm) of the culture filtrate measured starting from week-2 to week-8 compared to starting week-0, suggesting that biosurfactant was rapidly produced by CC within the first two weeks as a strategy to increase solubility of hydrophobic components of crude oil. In the presence of 0.1 and 1.0 mg/L of lead (Pb), degradation of crude oil was observed at 96% and 94%, respectively. In the case of cadmium (Cd), concentration at 0.01 mg/L resulted in 96% of crude oil degradation. However, at higher concentration of 0.1 mg/L Cd, only 73% of degradation was achieved. The CC was also shown able to remove Pb and Cd up to 90% from the culture media. The bacterial CC can adsorp metals onto the cell surfaces as shown using transmission electron microscopy (TEM). These results showed that the CC could be effective for bioremediation of crude oil waste contaminated with heavy metals