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Title: Stereological and Histopathological Study of Ovarian Tissue after Hydro-Alcoholic Extract of Artemisia Plants Compared with Metformin in Diabetic Rats
Authors: Kouhpeyma Farhad; Zahiri Shahla; Kargar Jahromi Hossein; Azhdari Sara; FarzamMohammad; Mahmoudi Teimourabad Saeid
Aff: 2Shiraz University of Medical Sciences, Department of Anatomy, Shiraz, Iran.
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Keywords: Ovarian Tissue;Histopathological Studies;Diabetic Rats;Artemisia;Metformin;Reproductive System;Metabolic Disorders;Periodic Acids
Abstract:Introduction: Diabetes is an endocrine and metabolic disorder, a variety of effects on the structure and function of many body systems leaves. Reproductive system, the rule is different. The aim of this study was to investigate the effects of the herb Artemisia extracts of ovarian tissue is improved due to diabetes complications. Materials and Methods: 50 rats, adult female aged approximately 10 weeks of Wistar rats randomly divided into 5 groups: control, diabetic (streptozotocin 50 mg/kg), diabetic + extract of Artemisia (200 & 300 mg/kg) and a positive control (metformin 250mg/kg) were included. The streptozotocin was administered intraperitoneally, and other materials as gavage. Treated mice continued for 8 weeks. At the beginning and end of the experiment, mice were weighed and blood glucose was measured, so the mice were dissected and the right ovary weighed after fixation in 3% formaldehyde and cutting and preparing for special studies were conducted respectively. Mason with three more chrome slides, were stained with hematoxylin eosin and periodic acid Schiff. Few studies include estimates of mean ovarian volume and follicle determines the quality and histopathological studies were performed. The results indicate an increase in blood sugar levels, ovarian volume, the percentage of atretic follicles quantitatively and cause congestion, hyperemia, and increased thickness of the germinal epithelium and fibrosis in diabetic rats was qualitatively, in the other groups, these parameters showed a decreasing trend (p = 0.05). Conclusion: This study suggests that as plant extract could significantly reduce the complications of diabetes in female reproductive system changes. Key words: diabetes, ovarian, moose, mountain sagebrush, stereology, histopathology