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Title:Toxicity Effects of Malathion, Dichlorvos and Temephos on Acetylcholinesterase in Climbing Perch (Anabas Testudineus) Authors: Asysyuura Adytia Patar; Wan Rozianoor Mohd Hassan; Nooraain Hashim ; Farida Zuraina Mohd Yusof DOI: Aff: University Teknologi MARA (UiTM), School of Biological Science, Faculty of Applied Sciences, 40450 Shah Alam Selangor, Malaysia. Author Email: Keywords: Anabas Testudineus; Malathion Dichlorvos; Temephos Acetylcholinesterase; Toxicity Effects URLs:ABSTRACT-HTML | FULLTEXT-PDF | Abstract:Malathion, dichlorvos and temephos pesticides are globally used for pest control in agricultural field. Widespread use of pesticides is a worldwide phenomenon. However, applications of pesticides are posing great danger to aquatic environment such as fish. The main objective of this study is to determine the acetylcholinesterase (AChE) activity in different tissues after exposure to malathion, dichlorvos and temephos. Total protein and enzyme activity was determined by Bradford, 1976 and Ellman et al, 1962 respectively. This study showed the optimum condition to be at pH 7 of phosphate buffer with 0.015 M of acetylthiocholine iodide (ACTHI) at 25 °C for 30 minutes. After exposure, the AChE activity decreased compared to the control in all fish organs exposed to high concentration of malathion (0.05 mg/L), dichlorvos (0.47 mg/L) and temephos (5.0 mg/L). However, the exposed fish recovered their AChE activity and the recovery was greater in liver, kidney and gill than in brain. It is concluded that exposure of pesticides in fish lead to inhibition of AChE activity which will cause physiological and biochemical disorders. Thus, the use of pesticides should be properly and strictly controlled and regulated to prevent indiscriminate use.